《表1 本研究用到的引物》

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《不同方向内含子对重组CHO细胞中神经生长因子表达的影响（英文）》

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Plasmid pCATG（constructed and preserved by our lab，pCAT-3 inserted G418 selection marker）was digested with XbaⅠand HindⅢ.The NGF gene was connected to a large fragment of pCATG and the new vector was named pNFG.The pNFG vector was digested with HindⅢ，and the resultant fragments were recovered.The recovered pNFG fragments as well as the forward and reverse introns obtained from PCR amplification were treated with alkaline phosphatase.The forward and reverse introns were added to 5?-upstream of NGF gene respectively.Vectors pNFGZ，containing the forward intron，and pNFGF，containing the reverse intron were constructed（Fig.1）.