《Table 1.Primers used in this study.》

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《Production of Two Elite Glutinous Rice Varieties by Editing Wx Gene》

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The Wx gene（LOC＿Os06g04200）was the target of Cas9endonuclease.According to the principles outlined in Ma et al（2015），two sgRNAs（T1 and T2）were designed on the first extron of the Wx gene from Nipponbare.The sgRNAs were assembled into one vector p YLCRISPR/Cas9-MH using Bsa I（New England Biolabs，Country）and T4 ligase（New England Biolabs，Country）.T1 and T2 were driven by Os U3 and Os U6a promoters，respectively.The vector was transformed into the Escherichia coli strain DH5α，and the insertions were verified by sequencing.The primers sequences of the sgRNAs are listed in Table 1.A vector map was generated using vector NTI（Lu and Moriyama，2004）.