《Table S1 Primers used in this study》

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《双头菌WH-1环氧乙烷二酸水解酶的克隆和酶学性质的研究（英文）》

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Genomic DNA of Labrys sp.WH-1 was extracted using an Ezup spin column bacterial genomic DNA isolation kit（Sangon Biothech，Shanghai，China）.ORCH was amplified with degenerate primers P1 and P2（Table S1），which were designed based on the sequences of the N-terminus and C-terminus of ORCH.The PCR products were purified and ligated with pUCm-T by using the T/A cloning procedure，and the recombinant plasmid was transformed into E.coli DH5αcells.A positive clone was sequenced.Primers P3 and P4（Table S1）were designed to amplify ORCH and cloned into vector pTrc99A with Nco I and Bam HI.ORCH was overexpressed in E.coli JM109 cells with addition of isopropyl thiogalactoside（IPTG）at 30°C for 18 h.