《Table 1 Information of primers used in this study》

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《Establishment of Real-time Fluorescent RT-LAMP Detection Method for Grouper Nervous Necrosis Virus》

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By using LAMP primer designing software Primer explorer V4，four specific primers were designed based on the ORF nucleotide sequences that encode CP gene（Gen Bank accession number:AY601879）of red spotted grouper nervous necrosis virus，including forward inner primer（FIP），backward inner primer（BIP），forward outer primer（F3），and backward outer primer（B3）.The primers were synthesized by Sangon Biotech（Shanghai）Co.，Ltd.The primer sequences were shown in Table 1.