《Table 1 Primers used in this study》

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《亚洲小车蝗触角转录组及化学感受蛋白基因表达谱分析（英文）》

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To validate the reliability of the sequences，genespecific primers were designed by Primer Premier 5.0to amplify the ORF sequences of all putative CSP genes（Table 1）.Total RNA was extracted from 40 antennae of male and female adults using TaKaRa Mini BEST Universal RNA Extraction Kit for first-strand cDNA synthesized by PrimeScriptTM1st Strand cDNA Synthesis Kit（Ta Ka Ra）according to the manufacturer’s instructions.The integrity and purity of RNA samples were measured using 1.5%agarose gel eletrophoresis and Nano PhotometerTMP-Class（IMPLEN，Germany），respectively.PCR reaction was programmed as follows:denaturation at 94℃for 3 min，followed by 30cycles of 94℃for 30 s，annealing at 60℃for 30 s（each primer uses a different annealing temperature），extension at 72℃for 60 s，and then incubation for 10min at 72℃.Amplified products were purified by agarose gel electrophoresis and cloned into pMD-19T vector（TaKaRa），then transformed into Escherichia coli DH5αcompetent cells（TaKaRa）.The white colonies were selected and placed in LB medium containing 100μg/m L ampicillin（Amp），oscillated overnight，and identified by PCR.The PCR products were sequenced for validation.