《Table 1 Primers used in this study》

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《芒果精氨酸脱羧酶基因MiADC的克隆及表达分析（英文）》

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The hexadecyl trimethyl ammonium bromide（CTAB）method was used for DNA and RNA extraction.The genomic DNA was precipitated using anhydrous ethanol，while the total RNA was deposited with lithium chloride（8 mol/L）.The first strand cDNA was synthesized by reverse transcription with Oligo（dT）18 Primers for homologous cloning and transcriptional analysis.The cDNA was submitted to the SMARTTM cDNA library construction kit（Clontech，USA）for clone of 3'and 5'RACE end of MiADC.Primers used for PCR or sequencing in this study were listed in Table 1，which were designed using the Primer Premier 5 software and synthesized via Sunbiotech Co.（Beijing，China）.Sequencing of these nucleotides was performed by using an automatic DNA sequencer ABI 3 700 from SinoGenoMax.