《Table 2–Primers used in this study.》

《Table 2–Primers used in this study.》   提示:宽带有限、当前游客访问压缩模式
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《Function of the auxin-responsive gene TaSAUR75 under salt and drought stress》


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Total RNA was isolated from different plant tissues using TRIzol reagent(Invitrogen,Carlsbad,CA,USA)according to the manufacturer's instructions and was treated with RNase-free DNase I(Fermentas,Vilnius,Lithuania).Next,c DNA was synthesized from 100 ng of total RNA using Revert Aid Reverse Transcriptase(Fermentas)and SYBR green q PCR Master Mix(Fermentas)was used for real-time PCR.The ACT2 gene of Arabidopsis was used to quantify the expression levels of Ta SAUR75 and the target gene in transgenic Arabidopsis plants.Ta Tubulin was used to quantify the expression levels of Ta SAUR75 in wheat.All primers used in this study are shown in Table 2.