《Table 2–Primers used in this study.》
本系列图表出处文件名:随高清版一同展现
《Function of the auxin-responsive gene TaSAUR75 under salt and drought stress》
Total RNA was isolated from different plant tissues using TRIzol reagent(Invitrogen,Carlsbad,CA,USA)according to the manufacturer's instructions and was treated with RNase-free DNase I(Fermentas,Vilnius,Lithuania).Next,c DNA was synthesized from 100 ng of total RNA using Revert Aid Reverse Transcriptase(Fermentas)and SYBR green q PCR Master Mix(Fermentas)was used for real-time PCR.The ACT2 gene of Arabidopsis was used to quantify the expression levels of Ta SAUR75 and the target gene in transgenic Arabidopsis plants.Ta Tubulin was used to quantify the expression levels of Ta SAUR75 in wheat.All primers used in this study are shown in Table 2.
图表编号 | XD0012318000 严禁用于非法目的 |
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绘制时间 | 2018.04.01 |
作者 | Yuan Guo、Qiyan Jiang、Zheng Hu、Xianjun Sun、Shoujin Fan、Hui Zhang |
绘制单位 | Institute of Crop Science, National Key Facility for Crop Gene Resources and Genetic Improvement, Chinese Academy of Agricultural Sciences、Institute of Crop Science, National Key Facility for Crop Gene Resources and Genetic Improvement, Chinese Academy of |
更多格式 | 高清、无水印(增值服务) |