《Table 2–Summary statistics of the simple sequence repeat (SSR) motifs designed with primers and dis

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《Development and validation of simple sequence repeat markers from Arachis hypogaea transcript sequences》

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To validate the identified novel SSR markers，we attempted to amplify the predicted SSRs via PCR.A total of 210 primer pairs（Table S2）were randomly selected for validation using DNA from 24 A.hypogaea varieties（Table S1）.The numbers of these selected SSRs with di-，tri-，and tetranucleotide repeats were 35，153，and 22，respectively.Among the 210 primer pairs，191（90.95%）were able to amplify genomic DNA and the containing SSRs with di-，tri-，and tetranucleotide repeats were 32（16.75%），140（73.30%），and 19（9.95%），respectively，whereas the remaining 19 primer pairs failed to generate PCR products at the same annealing temperatures（Table S2）.Most of the selected markers appeared as single alleles in all 24 A.hypogaea genotypes，except for a few multilocus SSRs，suggesting that these novel SSR markers possess a specific amplification in A.hypogaea.