《Table 1–Summary of EST-SSRs identified in transcriptome sequences.》

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《Development and validation of simple sequence repeat markers from Arachis hypogaea transcript sequences》

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All 128，725 unigenes assembled de novo from the A.hypogaea transcriptome（NCBI Sequence Read Archive database，SRP061959）with a total length of 98.47 Mb were used to identify potential SSR loci using MISA.A total of 29，357potential SSRs were identified in 22，806 unigenes，with 4883unigenes containing more than one SSR locus（Table 1）.The distribution density was one SSR locus per 3355 bp，and the number of repeat units ranged from one to six.The number of SSRs with each repeat motif varied widely.SSRs with mononucleotide repeat motifs were most abundant（19，065；64.94%），followed by tri-（5033；17.14%），di-（4927；16.78%），tetra-（303；1.03%），penta-（18；0.061%），and hexanucleotide（11；0.037%）repeat motifs（Table 1，Fig.S1）.Additionally，1710 SSRs were present in compound forms（Table 1）.The iteration number of repeat units in SSRs ranged from 4 to 22 and the occurrence frequencies of SSRs with different iteration numbers were unequal.The most common iteration number was 10（8467；28.84%），followed by 11（3959；13.49%），five（3634；12.38%）and six（3507；11.95%）（Table S3，Fig.S1） .For the SSRs with>10 repeat units，mononucleotide repeat motifs were most abundant，accounting for 99.82%of the SSRs，whereas motifs with>16 repeats were rare（5.79%）.The repetition of sequences also varied.Sixty-nine SSR motifs were identified，including two mono-，four di-，10 tri-，24tetra-，18 penta-，and 11 hexanucleotide repeating units（Table S3）.The dominant motif identified in the SSRs was A/T（18，358；62.54%），followed by AG/CT（2804；9.55%），AAG/CTT（1396；4.76%），AT/AT（1390；4.73%），AAT/ATT（1075；3.66%），ATC/ATG（725；2.47%）and AC/GT（720；2.45%）.The remaining62 motifs were relatively rare，accounting for only 9.84%of the total number of SSRs（Fig.1）.