《Table S1 DNA oligonucleotide sequences used in this study》

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《基于单链DNA开关调控的多功能生物电路（英文）》

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As illustrated in Fig.1A，the SSS is in silico designed as the hairpin structure（Ⅰ）consisting of five domains，in which domains b and b*，c and c*，d and d*hybridize together forming the stem of hairpin structure.A 20 nt single-stranded DNA trigger consisting of domains e*and d*can bind to the“toehold”region of SSS at the domain e of SSS.And the domain d*of trigger can further hybridize with domain d forcing SSS to become a second hairpin structure（Ⅱ），in which 3'end of domain c forms a stem structure for further DNA amplification.When Taq DNA polymerase was supplemented，DNA extended from domain c，eventually forming structure（Ⅲ）.During the conversion process from structure（Ⅱ）to structure（Ⅲ），the trigger will be removed by the highly processive 5'-3'exonuclease activity of Taq DNA polymerase（Taq DNAP）and the whole oneway reaction will be terminated.The dynamic structure change was detected by PAGE analysis.In the presence of SSS and Taq DNAP，the band of SSS（63 nt）migrated down.We suspected the reason was that the SSS was degraded by Taq DNA polymerase.In the presence of trigger molecule（20 nt），the band of structure（Ⅰ）migrated upward（Fig.1B），indicating that the structure（Ⅱ）has been formed by a specific hybridization between trigger and toehold region of SSS.After the Taq DNAP was added to SSS，the band of structure（Ⅱ）moved upward further and indicated that structure（Ⅲ）has been formed with the action of Taq DNAP（Fig.1B）.We also tested two complete in silico designed SSS sequences，Probe-1 and Probe-2（Supplementary Table S1），which effectively reacted with trigger molecule（Supplementary Fig.S1）.