《Table 2.Mutations and segregation analysis of the TKC1.2-LAZY1 T1 plants.》

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《Improvements of TKC Technology Accelerate Isolation of Transgene-Free CRISPR/Cas9-Edited Rice Plants》

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Protospacer adjacent motif site AGG is underlined.Zhonghua 11 plants were used as wild-type（WT）.‘+’refers to base pair insertion.‘-’refers to base pair deletion.Lowercase letter‘c’and‘g’in red refer to insertions of‘C’and‘G’，respectively.Num

All of the 126 TKC1.1-LAZY1 T1 plants had a mutation near the PAM site of the target sequence（Table 1）.Almost all of the73 TKC1.2-LAZY1 T1 plants were either homozygous or bi-allelic at the LAZY1 locus.However，we found two T1 plants from the#17 T0 plant that had a WT genotype（Table 2），despite that the#17 T0 plant had a lazy phenotype.Our results demonstrated that both TKC1.1 and TKC1.2 vectors were effective for generating targeted editing events.