《Table 1.Mutations and segregation patterns of TKC1.1-LAZY1 T1 plants.》

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《Improvements of TKC Technology Accelerate Isolation of Transgene-Free CRISPR/Cas9-Edited Rice Plants》

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WT，Wild-type Zhonghua 11 plant；HO，Homozygous mutation；HE，Heterozygous mutation；BI，Bi-allelic mutation.The underlined‘AGG’refers to the protospacer adjacent motif site required for Cas9 cleavage.‘-’refers to one base pair deletion.Lowercase letter‘

All of the 126 TKC1.1-LAZY1 T1 plants had a mutation near the PAM site of the target sequence（Table 1）.Almost all of the73 TKC1.2-LAZY1 T1 plants were either homozygous or bi-allelic at the LAZY1 locus.However，we found two T1 plants from the#17 T0 plant that had a WT genotype（Table 2），despite that the#17 T0 plant had a lazy phenotype.Our results demonstrated that both TKC1.1 and TKC1.2 vectors were effective for generating targeted editing events.