《Table 1.Development and analysis of T0 rice plants for targeted mutagenesis of Os LCT1 and OsNramp5

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《Characterization and Evaluation of OsLCT1 and OsN ramp5 Mutants Generated Through CRISPR/Cas9-Mediated Mutagenesis for Breeding Low Cd Rice》

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a Due to technical reasons，we failed to amplify the fragment encompassing the target site in exon 9（Fig.1-B）for the other plants.

A total of 124 HPT-positive T0 plants were raised from transformation with the three CRISPR/Cas9 vectors（Table 1）.Due to the difficulty in designing reliable primers for PCR amplification of the fragment encompassing the target site in exon 9 of Os Nramp5，a number of PCRs failed and consequently only four plants were included for mutation analysis of the 49T0 plants transformed with pH-nramp5×9（Table 1）.Two-thirds of the mutated pH-lct1×1 T0 plants were monoallelic heterozygous and the remaining mutants carried biallelic heterozygous mutations，i.e.two different mutations at the same target site（Table 1）.