《Table 1.CRISPR/Cas system used in rice.》
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《Development and Application of CRISPR/Cas System in Rice》
PAM，Protospacer adjacent motif.N represents A/T/G/C base.R represents A/G base.Y represents T/C base.V represents A/C/G base.
In addition，to improve the specificity of the CRISPR/Cas9 system，Zhang et al（2017）used three more specific Cas9 protein variants，eSpCas9（1.0），eSpCas9（1.1）and SpCas9-HF1，to achieve genome editing in rice.It has been demonstrated that the perfectly matched 20-nucleotide sgRNA sequences are necessary for high on-target editing efficiency and high fidelity of eSpCas9 and SpCas9-HF1（Table 1）.
| 图表编号 | XD0040097500 严禁用于非法目的 |
|---|---|
| 绘制时间 | 2019.03.28 |
| 作者 | REN Jun、HU Xixun、WANG Kejian、WANG Chun |
| 绘制单位 | State Key Laboratory of Rice Biology, China National Rice Research Institute, Chinese Academy of Agricultural Sciences、State Key Laboratory of Rice Biology, China National Rice Research Institute, Chinese Academy of Agricultural Sciences、State Key Laborat |
| 更多格式 | 高清、无水印（增值服务） |
