《Table 4 The results of variance analysis and significance analysis of regression model》

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《The Optimization of Ultrasonic-assisted Extraction of Polysaccharide from Sipunculus nudus by Response Surface Methodology and Study on Antioxidant Activity》

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Note:P<0.02:significant difference；P<0.005:highly significant difference；P<0.001:extremely significant difference.

Fitting of response surface test regression equationOn the basis of single factor test results，the extraction conditions of S.nudus polysaccharide were designed using software Design-Expert 8.05 with the extracting rate of S.nudus polysaccharide as response value，and the results are shown in Table 3.