《Table 2–Analysis of variance for BS resistance for significant markers detected by the BSA method i

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《Identification of stably expressed QTL for resistance to black shank disease in tobacco(Nicotiana tabacum L.) line Beinhart 1000-1》

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aMarker PT61373 and SNPCPS2showed no significant association with resistance to BS in BSA in this study，but the marker was tightly linked with a major QTL for resistance to BS in previous studies.bInformation for SNPCPS2obtained from Sallaud et al.[37].cG

The two major QTL，named q BS7 and q BS17，were repeatedly detected under different conditions in this study（Table 3and Fig.4）.The QTL q BS7 was mapped to the region between PT30174 and PT60621，and explained 17.40%–25.60%of the phenotypic variance under different conditions.The allele from BH increased resistance to BS.QTL q BS17 in the interval PT61564–PT61538 in linkage group 17 was detected in BC1F2in the field and in BC1F2:3in both the field and seedling stage tests，and explained 6.90%–11.60%of the phenotypic variance.The allele from XHJ decreased resistance to BS across all environments.