《Table 1.Candida albicans strains used in this study》

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《一种强力霉素诱导型白念珠菌基因敲除与筛选标记再循环工具包（英文）》

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Candida albicans strains used in this study are listed in Table 1.SN152 was kindly provided by Suzanne M.Noble[15].The other strains were constructed following the illustration of Figure 3.Simply，the loxP-CmLEU2-loxP cassette was amplified from pCPC48 with primers which were designed following the introduction of Figure 1-C.This cassette was introduced into SN152 to delete one copy of the target gene.loxP-CdHIS1-loxP was amplified from pCPC49 with the same primers and was introduced into the heterozygote to delete the second copy of the target gene，generating the homozygous null mutant.Then HACreH cassette was amplified from pCPC51 and was introduced into the mutant to perform marker excision by Dox induction.After excision，only one loxP site was left at the original locus for each copy.