《Table 1 Primers used for real-time PCR analysis》

《Table 1 Primers used for real-time PCR analysis》   提示：宽带有限、当前游客访问压缩模式
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《Characterization and allergic role of IL-33-induced neutrophil polarization》


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Abbreviations:CCL，C-C motif chemokine ligand；CXCL，C-X-C motif chemokine ligand；CCR，C-C motif chemokine receptor；CXCR，C-X-C motif chemokine receptor；HPRT，hypoxanthinephosphoribosyl transferase；IL，interleukin；IL-1R，interleukin-1 receptor.

Total RNA was isolated with TRIzol（Invitrogen，Carlsbad，CA，USA）and reverse transcription was performed with M-MLVsuperscript reverse transcriptase as described previously.Realtime PCR was performed using multiple kits（SYBR Premix Ex TaqTM，DRR041A，Takara Bio，Kusatsu，Japan）on CFX96（Bio-Rad，Hercules，CA，USA）.The used primers are summarized in Table 1.The m RNA expression of each gene was normalized to the housekeeping gene hypoxanthinephosphoribosyl transferase（HPRT）as reported previously.27The data are shown as the ratios of the interested genes to HPRT.For the inhibitor assay，cells were pretreated with various inhibitors for 30 min respectively，and then treated with IL-33 for 24 h.

图表编号XD0010736800    严禁用于非法目的
绘制时间2018.08.01
作者Bo Sun、Linnan Zhu、Yaling Tao、Hai-Xi Sun、Yang Li、Peng Wang、Yuzhu Hou、Yang Zhao、Xiaodong Zhang、Lianfeng Zhang、Ning Na、Yong Zhao
绘制单位State Key Laboratory of Membrane Biology,Institute of Zoology,Chinese Academy of Sciences、State Key Laboratory of Membrane Biology,Institute of Zoology,Chinese Academy of Sciences、State Key Laboratory of Membrane Biology,Institute of Zoology,Chinese Acade
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