《Table 1 Nucleotide sequence of mouse primers for real-time PCR amplification》
提示:宽带有限、当前游客访问压缩模式
本系列图表出处文件名:随高清版一同展现
《Expression and role of calcitonin gene-related peptide in mouse Aspergillus fumigatus keratitis》
PCR:Polymerase chain reaction;F:Forward;R:Reverse.
Real-time Polymerase Chain Reaction Total RNA in samples was separated by RNAiso plus reagent and quantified by spectrophotometry.The cDNA was synthesized by reverse transcription of total RNA(2μg)which was used in the following real-time polymerase chain reaction(PCR;20μL reaction volumes)with TB Green?Premix Ex Taq?II using specific primers:95℃for 30s,followed by 40 cycles of 95℃for 5s and 60℃for 30s and a final stage of 95℃for 15s,60℃for 30s,and 95℃for 15s.DNA contamination was assessed by melting curve.Different sets of target gene products were quantified by the 2ΔΔCT method[25].Table 1 lists are primer sequences in this study.
图表编号 | XD0051749600 严禁用于非法目的 |
---|---|
绘制时间 | 2019.05.18 |
作者 | Min Yin、Cui Li、Xu-Dong Peng、Gui-Qiu Zhao、Yuan Wu、Heng-Rui Zheng、Qian Wang、Qiang Xu、Nan Jiang |
绘制单位 | Department of Ophthalmology,the Affiliated Hospital of Qingdao University、Department of Ophthalmology,the Affiliated Hospital of Qingdao University、Department of Ophthalmology,the Affiliated Hospital of Qingdao University、Department of Ophthalmology,the A |
更多格式 | 高清、无水印(增值服务) |