《Table 1 Nucleotide sequence of mouse primers for real-time PCR amplification》

《Table 1 Nucleotide sequence of mouse primers for real-time PCR amplification》   提示:宽带有限、当前游客访问压缩模式
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《Expression and role of calcitonin gene-related peptide in mouse Aspergillus fumigatus keratitis》


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PCR:Polymerase chain reaction;F:Forward;R:Reverse.

Real-time Polymerase Chain Reaction Total RNA in samples was separated by RNAiso plus reagent and quantified by spectrophotometry.The cDNA was synthesized by reverse transcription of total RNA(2μg)which was used in the following real-time polymerase chain reaction(PCR;20μL reaction volumes)with TB Green?Premix Ex Taq?II using specific primers:95℃for 30s,followed by 40 cycles of 95℃for 5s and 60℃for 30s and a final stage of 95℃for 15s,60℃for 30s,and 95℃for 15s.DNA contamination was assessed by melting curve.Different sets of target gene products were quantified by the 2ΔΔCT method[25].Table 1 lists are primer sequences in this study.