《Table 1Primer sequences used for real-time quantitative PCR.》
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《Effect of high exposure of chlorogenic acid on lipid accumulation and oxidative stress in oleic acid-treated HepG2 cells》
Total RNA was extracted with Trizol reagent(Invitrogen,USA)after GCA treatment.RNA(3μg)was reverse transcribed to c DNA using Reverse Transcription kit(Toyobo,China)and amplified using SYBR Green I q PCR kit(Bio-Rad,USA).Data were normalized toβ-actin expression and further normalized to control.Primers used for SREBP-1c and PNPLA3 were shown in Table 1.
图表编号 | XD007995700 严禁用于非法目的 |
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绘制时间 | 2018.04.18 |
作者 | Ya-yun Liu、Ting Zhai、Qing-qing Yu、Jing Zhu、Yong Chen |
绘制单位 | Key Laboratory of Biotechnology of Chinese Traditional Medicine of Hubei Province, National & Local Joint Engineering Research Center of High-throughput Drug Screening Technology, Hubei University、Key Laboratory of Biotechnology of Chinese Traditional Med |
更多格式 | 高清、无水印(增值服务) |