《Table 1Primer sequences used for real-time quantitative PCR.》

《Table 1Primer sequences used for real-time quantitative PCR.》   提示:宽带有限、当前游客访问压缩模式
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《Effect of high exposure of chlorogenic acid on lipid accumulation and oxidative stress in oleic acid-treated HepG2 cells》


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Total RNA was extracted with Trizol reagent(Invitrogen,USA)after GCA treatment.RNA(3μg)was reverse transcribed to c DNA using Reverse Transcription kit(Toyobo,China)and amplified using SYBR Green I q PCR kit(Bio-Rad,USA).Data were normalized toβ-actin expression and further normalized to control.Primers used for SREBP-1c and PNPLA3 were shown in Table 1.