《Tab.1 Sequences of the primers for amplifying the genes using real-time RT-PCR》

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《地塞米松对内毒素诱导的小鼠葡萄膜炎的治疗效果及转录组分析（英文）》

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The total RNA of neural retina was extracted with Trizol Reagent（Invitrogen，Carlsbad，CA，USA）following the manufacturer&apos；s instructions.For quantitative PCR，c DNA was synthesized using the Prime Script RT reagent kit（TakaraBiotechnology，Dalian，China）.PCR amplification was performed using a real-time PCR system（ABI 7500 system，Applied Biosystems，Foster City，CA，USA）with SYBR Green（Vazyme Biotech Co.，Ltd，Nanjing，China）.The thermal cycles were carried out with initial denaturation at 95℃for 10 min，followed by40 cycles of 95℃for 15 s and 60℃for 1 mi.To determine the m RNA levels，GAPDH was used as the endogenous reference gene.All the samples were prepared in duplicate and the average Ct values were used for quantification.Relative quantification was achieved using the comparative 2-ΔΔCtmethod.The control group was used as the calibrator to calculate the relative fold change of the m RNA of the target gene X in the experimental group（EG）:ΔΔCt=（Ct.XEG-Ct.GAPDHEG）/（Ct.Xcontrol-Ct.GAPDHcontrol）[21].The sequences of primers and Gen Bank accession numbers of the target genes are listed in Tab.1.