《Table 1.Primer Sequences Used for Real-Time PCR》

《Table 1.Primer Sequences Used for Real-Time PCR》   提示：宽带有限、当前游客访问压缩模式
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《Garlicin Post-conditioning Suppresses Adhesion Molecules in Porcine Model of Myocardial Ischemia-Reperfusion Injury》


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Three hours after reperfusion，swine hearts were excised and the left ventricles were sectioned into10-mm-thick cross-sectional myocardial slices.Among the myocardial slices，the cross-section at the left ventricular papillary muscle level was chosen.Normal area，RA and NRA were then frozen and crushed in liquid nitrogen.Total RNA was then extracted with RNA extraction kit（TaKaRa，Japan）as per the manufacturer's guidelines and quantified by absorbance at 260 nm.The reaction volume of polymerase chain reaction（PCR）was set at 10μL，while the reaction temperature，for a total of 40 cycles，was fixed at 95℃for 5 s and 60℃for 30 s.Primer sequences used for this procedure are depicted in Table 1.β-Actin was used as the internal standard.PCR amplification was performed in a volume of 10μL containing 2μL of the cDNA template，2×SYBR?Premix Ex TaqTm（TaKaRa，Japan）5μL，primers（5μmol/L，forward and reverse each，Invitrogen，USA）and RNase free d H2O 1μL.

图表编号XD0049341600    严禁用于非法目的
绘制时间2019.01.01
作者YANG Peng、LI Jia-hui、LI Ai-li、LI Jing、WANG Yong、REN Shi-yan、LI Xian-lun
绘制单位Department of Cardiology, China-Japan Friendship Hospital、Department of Cardiology, China-Japan Friendship Hospital、Department of Cardiology, China-Japan Friendship Hospital、Department of Cardiology, China-Japan Friendship Hospital、Department of Cardiolog
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