《Table 2 The primers used for real-time RT-PCR》
本系列图表出处文件名:随高清版一同展现
《Effects of stocking density on the growth and immunity of Atlantic salmon salmo salar reared in recirculating aquaculture system(RAS)》
F:forward primer;R:reverse primer.
About 5 872 of Atlantic salmon with an average body mass of 1 413.1±52.3 g were purchased from Shandong Oriental Ocean Sci-Tech Co.,Ltd.Shandong Province,China.The fi sh were distributed to the 5 experimental RAS tanks(6 m×6 m×3 m)randomly.The initial density of each tank is,A:24 kg/m 3;B:21 kg/m 3;C:15 kg/m 3;D:9 kg/m 3 and E:6 kg/m 3.Experimental trials were conducted for220 days.During the experimental period,water temperature was maintained at 15.0±0.7°C,pH 7.2–7.5,total ammonia-nitrogen<0.25 mg/L,salinity between 28.0±0.5.Water was supplied to each tank at a rate of approximately 50 L/min with liquid oxygen to maintain dissolved oxygen between 130±10%saturation in the tank.Illumination was continuous and provided by one fl uorescent tube light per tank with light intensity of 60±10 lux on the surface of water.Fish were fed with a commercial salmon diet(Skretting,Norway)containing 42%protein and 22%fat at satiation level three times a day by fi sh automatic feeder(at about 6:00 AM,14:00 PM and 20:00 PM)during the period of light-manipulation.The stocking density variations were showed in Table 1.All procedures described in this study were reviewed and approved by the ethical committee of Institute of Oceanology,Chinese Academy of Sciences.
图表编号 | XD0034435000 严禁用于非法目的 |
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绘制时间 | 2019.01.01 |
作者 | WANG Yanfeng、CHI Liang、LIU Qinghua、XIAO Yongshuang、MA Daoyuan、XIAO Zhizhong、XU Shihong、LI Jun |
绘制单位 | CAS Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences、Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology、Center for Ocean Mega-Science, Chinese A |
更多格式 | 高清、无水印(增值服务) |