《Table 1 Primers used in real-time PCR for Kyoho grape (Vitis vinifera×Vitis labrusca)》

《Table 1 Primers used in real-time PCR for Kyoho grape (Vitis vinifera×Vitis labrusca)》   提示：宽带有限、当前游客访问压缩模式
本系列图表出处文件名：随高清版一同展现
《Effects of different color paper bags on aroma development of Kyoho grape berries》


  1. 获取 高清版本忘记账户？点击这里登录
  1. 下载图表忘记账户？点击这里登录

Real-time PCR analysis of LOX-HPL pathway genes was performed with SYBR Green SuperReal PreMix（Tiangen，Beijing，China）according to the instruction using CFX Connect?Real-Time PCR Detection System（Bio-Rad，Hercules，CA，USA）.Each reaction contained10μL 2×SuperReal PreMix Plus，1μL cDNA，0.6μL forward primer，0.6μL reverse primer and added ddH2O to 20μL.The real-time qPCR program used was 95°C for 15 min followed by 40 cycles of amplification with 95°C for 10 s，60°C for 32 s，followed by a melt cycle from 60 to 95°C.The primers used in this study were shown in Table 1.GAPDH and Ef1-αwere taken as internal controls.For all analyses，the signal obtained for target genes was normalized against the mean of cycle threshold（Ct）values of two reference genes，and relative quantification of target genes was calculated by 2–ΔΔCt method.All samples were tested in three biological replicates.

图表编号XD0047425200    严禁用于非法目的
绘制时间2019.01.20
作者JI Xiao-hao、WANG Bao-liang、WANG Xiao-di、SHI Xiang-bin、LIU Pei-pei、LIU Feng-zhi、WANG Hai-bo
绘制单位Research Institute of Pomology,Chinese Academy of Agricultural Sciences、Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (Germplasm Resources Utilization),Ministry of Agriculture、Research Institute of Pomology,Chinese Academy of Ag
更多格式高清、无水印（增值服务）

相关图表

  1. 《Table 1 Nucleotide sequence of mouse primers for real-time PCR amplification》2019.05.18
  2. 《Table 3 Primers used for real-time quantitative PCR》2019.01.20
  3. 《Table 1 Sequences of primers used in quantitative real-time RT-PCR》2019.01.01
  4. 《Table 2 The primers used for real-time PCR》2019.02.20
  5. 《表1 实时定量PCR使用的引物序列Tab 1 Oligonucleotide primers sequences used for real-time PCR》2019.03.28
  6. 《表1 实时定量PCR上、下游引物Tab.1 The primers used in real-time qPCR》2019.01.28
  7. 《表2 基于细菌16S r DNA的引物及扩增产物的退火温度和片段大小Tab.2Specific primers used for real-time PCR》2019.01.28
  8. 《Table 2 The primers used for real-time RT-PCR》2019.01.01
  9. 《Table 1 Genes and primers used in the quantitative real-time PCR analysis》2019.01.01
  10. 《Table 1 Primers used in methylation-specific PCR and real-time PCR analysis》2019.01.18
  11. 《表3 mi RNA荧光定量引物Tab.3 Primers used for mi RNA and m RNA quantitative real-time PCR (q PCR)》2018.11.28
  12. 《表2 荧光定量引物Tab.2 Primers used for real-time quantitative PCR analyze》2018.04.01
  13. 《Tab.1 Sequences of the primers for amplifying the genes using real-time RT-PCR》2018.08.20
  14. 《表1 RT-PCR中内参及目的基因引物序列Tab.1 Primers and probes used for real-time PCR》2018.03.20
  15. 《表1 实时荧光定量PCR所用基因的特异性引物及其序列Tab.1 Specific primers and its sequences used for real-time PCR》2018.07.01
  16. 《Table 1 Genes and specific primers used for real-time polymerase chain reaction》2018.12.25
  17. 《表2 实时定量PCR引物Tab.2 Primers used for quantitative real-time PCR》2018.03.25
  18. 《Table 2 Primers for real-time PCR》2018.08.01
  19. 《Table 1 Primers used for real-time PCR analysis》2018.08.01
  20. 《表1 基因克隆及实时荧光定量PCR中使用的引物Tab.1 Primers used for cloning and real-time quantitative PCR》2018.07.01

随机翻阅

《表1 胶东半岛黄金矿山进路胶结充填体配比设计》 《表4 Box-Behnken设计方案及响应值》 《表1 两种方案优缺点对比》 《表1 流动相梯度洗脱程序表》 《表1 FDA收录的眼用制剂品种规格及推荐的生物等效性评价方法[3-39]》 《表2 时态用法错误频数表》 《表1 明清入连山的外籍姓氏统计汇总表》 《表4 上海、江苏、福建3省市中职学考考试组织形式、时间、次数》 《表4：广播电视定向催费系统研究与设计》 《表4 脉冲紫外激光改性前后PPS表面不同价态的硫元素相对含量Tab.4 Relative content of sulfur element on PPS surfaces before and after pulsed UV laser》