《Table 1 Primers used in methylation-specific PCR and real-time PCR analysis》

  提示：宽带有限、当前游客访问压缩模式

本系列图表出处文件名：随高清版一同展现

《5-aza-2'-deoxycytidine in the regulation of antioxidant enzymes in retinal endothelial cells and rat diabetic retina》

1. 获取 高清版本忘记账户？点击这里登录

1. 下载图表忘记账户？点击这里登录

Gene Expression Primary cultured HRECs and rat retinas were used to extracted total RNA by Trizol（Invitrogen Life Techologies，Grand Island，NY，USA）according the manufacturer’s instructions.The RNA was reverse transcribed applying the Takara First Strand Synthesis kit（TaKaRa，Dalian，China）to synthesis cDNA.Real-time quantitative PCRwas performed using SYBR Green PCR kit（TaKaRa，Dalian，China）on an ABI Prism 7000 system.PCR procedure was set as the following:denaturing at 95℃for 5min，followed by 40 cycles of denaturation at 95℃，annealing at 60℃，and extension at 72℃for 10s，respectively.The sequences of the primers used in the experiments are summarized in Table 1.