《Table 1 Primer sequences used for genotyping SNPs》
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《中国中原地区汉族人群染色体4q25上rs17042171与心房颤动有关（英文）》
M:Mutant；W:Wild；C:Common；F:Forward；R:Reverse
G e n o m i c D N A w a s e x t r a c t e d f r o m E D TA-anticoagulated peripheral blood leukocytes using the Genomic DNA kit（Tiangen Biotech Co Ltd，Beijing，China）according to the manufacturer’s protocol and stored at–20℃.We used ARMS-PCR to genoty pe rs17042171，rs6771157 and rs337711.Direct sequencing for rs6795970 and rs11264280 was applied.Primers were designed by primer 5.0 software and listed in Table 1.A20μL reaction system was used for PCR amplification，including 10μL of 2×PCR MIX，7μL of sterile water，2μL of genomic DNA，and 1μL of primer.The following PCR conditions were employed:For rs17042171 and rs6795970，94℃for 4 min，30 cycles of 94℃for 30 s，60℃for 30 s，and 72℃for 30 s，and then 72℃for7 min.For rs6771157，94℃for 4 min，30 cycles of 94℃for 30 s，45℃for 30 s，and 72℃for 30 s，and then 72℃for 7 min.For rs337711，94℃for 4 min，30 cycles of 94℃for 30 s，50℃for 30 s，and 72℃for 30 s，and then 72℃for 7 min.For rs11264280，94℃for 4 min，30 cycles of94℃for 30 s，52℃for 30 s，and 72℃for 30 s，and then72℃for 7 min.PCR products were analyzed by agarose gel electrophoresis，one tenth of which were randomly selected for sequencing to ensure the quality of the genotyping of rs17042171，rs6771157，and rs337711.
| 图表编号 | XD0026941300 严禁用于非法目的 |
|---|---|
| 绘制时间 | 2018.06.01 |
| 作者 | 王旭、聂亚莉、宁姝威、师勇、赵育洁、牛思泉、郭成贤、孟祥光、袁义强 |
| 绘制单位 | 南方医科大学附属河南心血管病医院心内科、郑州大学基础医学院药理学教研室、郑州市第七人民医院心血管疾病及药物研究室、郑州市第七人民医院泌尿外科、郑州市第七人民医院心内科、郑州市第七人民医院心内科、中南大学湘雅三医院临床药理研究中心、郑州市第七人民医院心血管疾病及药物研究室、南方医科大学附属河南心血管病医院心内科 |
| 更多格式 | 高清、无水印（增值服务） |
