《Table 1 Primers for PCR analysis》

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《异源表达芸薹生链格孢谷氨酸脱氢酶基因AbGDH提高水稻氮素利用率的研究（英文）》

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Note:The underlined sequences indicate the restriction enzyme Bam HⅠcleavage site.

The full-length cDNA of AbGDH gene was isolated from the A.brassicicola using reverse transcription PCR with degenerate primers（Table 1）according to the method described by Zhou et al[14].The PCR product was verified by sequencing and cloned into the GATEWAY donor vector pGWC[29].Then，the full-length coding sequence of AbGDH was subcloned into the binary vector pCAMBI-A1301GW（a slightly modified pCAMBIA1301 vector）via LR reaction as described previously[14].The resulting vector was named pCAMBIA1301GW-AbGDH.