《Table 1Primers for RT-PCR.》

《Table 1Primers for RT-PCR.》   提示：宽带有限、当前游客访问压缩模式
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《Toxicity-efficacy converting of ginseng combined with Fuzi Banxia incompatibility in heart failure stage of cor pulmonale》


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Total RNA of right ventricular myocardium were extracted using RNAsimple total RNA kit（Tiangen Biotech Co.，Ltd，Beijing，China）and reverse transcribed into c DNA with Fast Quant RT kit（with g DNase）according to the manufacturer’s instructions for subsequent analysis of the m RNA levels of myocardial hypertrophyrelated and apoptosis-related genes including natriuretic peptide A（ANP），B-type natriuretic peptide（BNP），beta myosin heavy chain（β-MHC），B-cell lymphoma 2（Bcl-2），and Bcl-2-associated X protein（Bax）were determined by using Super Real Pre Mix Plus（SYBR Green）in a 20μL reaction volume.All PCR reactions were done in triplicate.GAPDH served as an endogenous control.Targeted genes（Table 1）were chosen according to literatures（Ding et al.，2016；Shi et al.，2016）.

图表编号XD007992600    严禁用于非法目的
绘制时间2018.04.18
作者Ying-ying Huang、Xiao-jin Li、Kai-xin Liu、Peng-wei Zhuang、Yan-jun Zhang
绘制单位College of Chinese Materia Medica, Tianjin University of Traditional Chinese Medicine、College of Chinese Materia Medica, Tianjin University of Traditional Chinese Medicine、School of Pharmaceutical Sciences, Zhengzhou University、College of Chinese Materia
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