《Table 1 Primers used for genetic analysis and band sizes obtained》

《Table 1 Primers used for genetic analysis and band sizes obtained》   提示:宽带有限、当前游客访问压缩模式
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《"Influence of polymorphisms in VEGF, ACE, TNF and GST genes on the susceptibility to retinopathy of prematurity among Chinese infants"》


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Genetic Analysis Genetic analysis was performed on the blood specimens of the subjects.VEGF-460T/C polymorphism was genotyped by sequencing method and the primers used were shown in Table 1.The other three VEGF polymorphisms(-634G/C,+405G/C and+936C/T),along with TNF-308G/A and GSTP1 Ile/Val polymorphisms were genotyped by PCR-RFLP method and the results were validated by performing DNA sequencing on 10%of the samples.The primers used were also shown in Table 1.The VEGF-634G/C and+405G/C polymorphisms were both digested with Bsm FI restriction enzyme,while the remaining three polymorphisms were digested with Nla III,Nco I and Alw26I respectively.The band sizes obtained were used for categorization of genotypes as shown in Table 1.Besides,ACE ID,GSTT1 null/present and GSTM1 null/present polymorphisms were genotyped by PCR method.Results of ACE ID polymorphism was confirmed using a second PCR,while results of GSTT1 and GSTM1polymorphisms were confirmed by amplification of albumin gene as an internal control.All primers used and the band sizes obtained are shown in Table 1.