《Table 1 Primers used for genetic analysis and band sizes obtained》
本系列图表出处文件名:随高清版一同展现
《"Influence of polymorphisms in VEGF, ACE, TNF and GST genes on the susceptibility to retinopathy of prematurity among Chinese infants"》
Genetic Analysis Genetic analysis was performed on the blood specimens of the subjects.VEGF-460T/C polymorphism was genotyped by sequencing method and the primers used were shown in Table 1.The other three VEGF polymorphisms(-634G/C,+405G/C and+936C/T),along with TNF-308G/A and GSTP1 Ile/Val polymorphisms were genotyped by PCR-RFLP method and the results were validated by performing DNA sequencing on 10%of the samples.The primers used were also shown in Table 1.The VEGF-634G/C and+405G/C polymorphisms were both digested with Bsm FI restriction enzyme,while the remaining three polymorphisms were digested with Nla III,Nco I and Alw26I respectively.The band sizes obtained were used for categorization of genotypes as shown in Table 1.Besides,ACE ID,GSTT1 null/present and GSTM1 null/present polymorphisms were genotyped by PCR method.Results of ACE ID polymorphism was confirmed using a second PCR,while results of GSTT1 and GSTM1polymorphisms were confirmed by amplification of albumin gene as an internal control.All primers used and the band sizes obtained are shown in Table 1.
图表编号 | XD0023066100 严禁用于非法目的 |
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绘制时间 | 2018.09.18 |
作者 | Xiao-Jun Lei、Yong-Xia Zhao、Tong Qiao |
绘制单位 | Department of Ophthalmology,Friendship Hospital of Dalian、Department of Ophthalmology,Center Hospital in Cangzhou、Department of Ophthalmology,Shanghai Children's Hospital,Shanghai Jiao Tong University |
更多格式 | 高清、无水印(增值服务) |