《Table 2.PCR primers used for plasmid construction, cassettes amplification and verification》

《Table 2.PCR primers used for plasmid construction, cassettes amplification and verification》   提示:宽带有限、当前游客访问压缩模式
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《一种强力霉素诱导型白念珠菌基因敲除与筛选标记再循环工具包(英文)》


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TcxP sites were shown in italic and bold.

C.albicans are directly transformed with PCR products containing loxP-marker-loxP with 78 bp flanking homology region from target genes without purification.The transformation was performed by following an improved protocol[26].All mutants were validated via diagnostic PCR using gene-specific primers and universal primers VP9&VP10 for CmLEU2,VP11&VP12 for CdHIS1,VP13&VP14for CdARG4(Table 2).