《Table 1–Primers used for tracing chromosomes in homoeologous group 2 of wheat, barley, and Th.inter

《Table 1–Primers used for tracing chromosomes in homoeologous group 2 of wheat, barley, and Th.inter   提示:宽带有限、当前游客访问压缩模式
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《Development and genetic analysis of wheat double substitution lines carrying Hordeum vulgare 2H and Thinopyrum intermedium 2Ai#2 chromosomes》


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Primers specific to barley chromosome 2H were designed based on the sequences of wheat ESTs located on wheat group2 chromosomes using Primer Premier 6.0 software(http://www.premierbiosoft.com/primerdesign/).Primers specific to the alien chromosome Th.intermedium 2Ai#2 and to the wheat chromosomes 2A,2B,and 2D,designed based on the sequences of TaARG genes located on wheat group 2 chromosomes,were developed in our previous studies[28,29].Primers P1-2A,P2-2B,and P3-2D specifically identify wheat chromosomes 2A,2B,and 2D,and primers P97 and P79 can be used for specific detection of Th.intermedium chromosome2Ai#2 and barley chromosome 2H at the same time by amplifying bands of different size(Table 1).