《Table 2–Identification of plants in the F1to F5generations containing different combinations of chr

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《Development and genetic analysis of wheat double substitution lines carrying Hordeum vulgare 2H and Thinopyrum intermedium 2Ai#2 chromosomes》

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Six crosses，N431/X99-7，N452/X99-7，X99-12/N420，X99-12/N439，X99-4/N420，and X99-4/N439，corresponding to F1codes A，B，C，D，E，and F，respectively，were made and the progeny were repeatedly selfed.Molecular markers that had been previously verified in different materials including wheat and different wheat substitution lines were used to genotype the plants in the F1to F5generations（Tables 1，2）.To verify that the F1plants from the cross combinations were genuine hybrids，two molecular markers，P79 and P97，which specifically amplify fragments from the long arm of barley2H and the short arm of Th.intermedium 2Ai#2，respectively，were employed to genotype all the F1plants.The F1plants from five of the six cross combinations were found to carry both alien chromosomes.The F1plants from the remaining cross combination，N431/X99-7（A），carried chromosome2Ai#2 but not 2H（Fig.1）and were excluded from further experiments.