《Table 1 Identification of differentially expressed proteins in silkworm near-isogenic line of the d

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《谷胱甘肽硫转移酶参与家蚕氟化物耐受性形成的研究》

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Image Master 2D Platinum was adopted to analyze the differential 2D profiles.Eight obviously different protein spots were taken from the 2D gels to undergo trypsin enzymolysis，extraction of peptide，and desalting treatment by Zip Tip tips，and then they were sent to Shanghai Applied Protein Technology Co.，Ltd.（China）for identification with matrix-assisted laser desorption ionization-time of flight mass spectrometry（MALDI-TOF-MS）.The peptide mass fingerprinting（PMF）signal of the five protein spots except protein spots 1，6 and 8 was obvious，and the signal-to-noise ratio was high.After PMF analysis with the software and retrieval in the NCBI database and in the silkworm genomic database（Silk DB），the identification results are obtained，as illustrated in Table 1.