《Table 2 Identification of the differentially expressed spots by mass spectrometry (MS) 1)》

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《"GsMAPK4,a positive regulator of soybean tolerance to salinity stress"》

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1) MW，molecular weight；pI，protein isoelectric.2) GI，geninfo identifier.

Total RNA was extracted，and the quantitative real-time PCR（qRT-PCR）analysis was performed according to a previous report by Huang Y L et al.（2013）.Actin was used as a reference gene in this study.Primer information is shown in Table 1.The qRT-PCR reaction was performed at 95°C for 10 s，followed by 40 cycles at 95°C for 5 s，60°C for 31 s by using the two-step RT-PCR.RT-PCR analysis was performed by the 2–ΔΔCT method（Livak and Schmittgen 2001）.