《Table 1 Primers for overlapping extension PCR from pcDNA4.1/APP695》
提示:宽带有限、当前游客访问压缩模式
本系列图表出处文件名:随高清版一同展现
《Mutations of beta-amyloid precursor protein alter the consequence of Alzheimer's disease pathogenesis》
Wild-type human APP695(APP-WT)and beta-secretase 1(BACE1)-myc-his plasmids were obtained from the Weihong Song Lab(University of British Columbia,Vancouver,Canada).Mutants of APP(specifically,A673T,A673V,E682K,E693G,and E693Q)were amplified by overlapping extension polymerase chain reaction(PCR)from pcD-NA4.1/APP695 with appropriate primers(Table 1).PCR fragments were then subcloned into pcDNA4.1 vector with Hind III and Xba I.
图表编号 | XD0040613000 严禁用于非法目的 |
---|---|
绘制时间 | 2019.04.01 |
作者 | Nuo-Min Li、Ke-Fu Liu、Yun-Jie Qiu、Huan-Huan Zhang、Hiroshi Nakanishi、Hong Qing |
绘制单位 | School of Life Science, Beijing Institute of Technology、School of Life Science, Beijing Institute of Technology、School of Life Science, Beijing Institute of Technology、School of Life Science, Beijing Institute of Technology、Department of Aging Science and |
更多格式 | 高清、无水印(增值服务) |