《Table 1 Primers for overlapping extension PCR from pcDNA4.1/APP695》

《Table 1 Primers for overlapping extension PCR from pcDNA4.1/APP695》   提示:宽带有限、当前游客访问压缩模式
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《Mutations of beta-amyloid precursor protein alter the consequence of Alzheimer's disease pathogenesis》


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Wild-type human APP695(APP-WT)and beta-secretase 1(BACE1)-myc-his plasmids were obtained from the Weihong Song Lab(University of British Columbia,Vancouver,Canada).Mutants of APP(specifically,A673T,A673V,E682K,E693G,and E693Q)were amplified by overlapping extension polymerase chain reaction(PCR)from pcD-NA4.1/APP695 with appropriate primers(Table 1).PCR fragments were then subcloned into pcDNA4.1 vector with Hind III and Xba I.