《表1 用于半定量RT-PCR和qRT-PCR的引物》

  提示：宽带有限、当前游客访问压缩模式

本系列图表出处文件名：随高清版一同展现

《Bmo-miR-2788对家蚕丝胶蛋白基因Ser-1的表达调控（英文）》

1. 获取 高清版本忘记账户？点击这里登录

1. 下载图表忘记账户？点击这里登录

BmSer-1 3′-UTR fragment has a length of about300 bp amplified from the genomic DNA of silkworm by PCR.PCR cycling conditions were as follows:initial denaturation at 94℃for 4 min，25 cycles of 94℃for 30 s，55℃for 30 s and 72℃for 45 s，and final extension at 72℃for 5 min.PCR products were separated by electrophoresis with 1%agarose gel.After purification，the gene fragment containingBmSer-1promoter，egfpORF andBmSer-1 3′-UTR was cloned into pMD19-T vector and sequenced.InsertedBmSer-13′-UTR fragment in pMD19-T was cleaved byXbaⅠandFseⅠand ligated into pGL3.0（A3-luc-SV40）plasmid（previously digested by the same restriction enzymes）to construct pGL3.0（A3-luc-BmSer-1-3′-UTR-SV40）.In order to construct Bmo-miRNA expression vector pcDNA3.0（ie1-egfp-pri-Bmo-miR-2788-SV40），nucleotide sequences of mature Bmo-miRNA，BmomiR-2788 and their flanking regions（±100 bp)were cloned into the pCDNA3.0 plasmid downstream ofegfp gene controlled byB.morinucleopolyhedrovirus（BmNPV）ie1 promoter.Then，structure of obtained vectors was confirmed by sequencing（Sangon Biotech）.