《Table 3 Total and sn-2 fatty acid compositions of the raw tuna oil》

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《Preparation of DHA-and EPA-enriched Glycerides by Enzymatic Interesterification Using Tuna Oil and Capric Acid》

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Note:aonly the major fatty acids are listed in the table；beach value represents the mean of three determinations±standard deviation；cvalues are obtained according to the 1，3-random-2-random hypothesis；dU and S are unsaturated and saturated fatty acids，r

On the basis of single-factor experiments，response surface methodology was used to investigate the effect of four factors on Y（DHA+EPA content in glycerides）after acidolysis.A Box-Behnken design（BBD）with four factors and three levels was used to generate factor combinations by using the Design-Expert 8.0.5.0（Stat-Ease Inc.，Minneapolis，MN，USA）software.The four factors were A（reaction temperature，oC），B（substrate molar ratio，capric acid/tuna oil），C（enzyme amount，based on the total substrate，%），and D（reaction time，h）.The levels of the four factors based on the single-factor experiments are listed in Table 1.The experimental design with observed values is shown in Table 2.A total of 29 experimental runs was gene rated.