《Table 1 Amino acid composition of Os PT2 protein》

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《Analysis of Protein Characteristics of Selenite Transporter OsPT2 in Rice》

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*is hydrophobic amino acid，and#is hydrophilic amino acid.

The amino acid sequence of rice selenite transporter Os PT2had a total length of 528 aa.Its physical and chemical properties were analyzed with Prot Param tool，and the results showed that the protein had the relative molecular weight of 57.815 k D，and the theoretical isoelectric point p I was 8.85.The amino acid composition is shown in Table 1.Among the various amino acids，the negatively-charged aspartic acid（Asp）and glutamic acid（Glu）had a total number of 32，accounting for 6.06%，and the positivelycharged arginine（Arg）and lysine had a total number of 38，accounting for 7.20%.The molecular formula of Os PT2 was C2677H4072N672O707S26，consisting of 8 154 atoms.Its instability index was 30.53，lower than 40，suggesting that Os PT2 is a protein with better stability.The fat-soluble index of the protein was 93.22，and the average hydrophilic coefficient was 0.398，so the protein was a hydrophobic protein.The distribution of its hydrophilic/hydrophobic amino acids was further analyzed with Prot Scale.As shown in Fig.1，there were 165 hydrophilic amino acids，accounting for 31.25%，and there were 297 hydrophobic amino acids，accounting for 56.25%.