《Table 1 Binding affinity of hPEBP4-derived peptides to HLA-A*0201 molecules》

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《A modified HLA-A~*0201-restricted CTL epitope from human oncoprotein (hPEBP4) induces more efficient antitumor responses》

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Abbreviations:hPEBP4，human phosphatidylethanolamine-binding protein 4；MFI，mean fluorescence intensity.aPropred-I prediction software，available at http://crdd.osdd.net/raghava//propred1/.bPeptide binding was evaluated by the MFI.SSp-1（RLNEVAKNL）and OVA

To determine whether h PEBP4 has the potential to elicit an immunogenic response in the host，we screened the h PEBP4 amino-acid sequence using several well-established MHC-binding peptide prediction algorithms that assist in the identification of novel T-cell epitopes.We then synthesized 10predicted nonameric peptides with the highest estimated half-life of dissociation from HLA-A*0201 and tested their affinity binding to HLA-A*0201 using the TAP-deficient T2 cellpeptide test（Table 1）.Of the 20 candidate peptides，No.2（P12–20），No.3（P40–48）and No.6（P6–14）were high-affinity epitopes（FI=1.15，1.59 and 1.04，respectively）；all others were low-affinity epitopes（FIo1；Table 1）.The positive control SSp-1exhibited strong binding to HLA-A*0201（FI=1.65），12whereas the negative control exhibited no binding（FI=0.03），as expected.