《Table 1 Oligonucleotide primers for real-time polymerase chain reaction》

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《Early constraint-induced movement therapy affects behavior and neuronal plasticity in ischemia-injured rat brains》

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NgR:Nogo-receptor；BDNF:brain-derived neurotrophic factor；NGF:nerve growth factor.

TRIzol reagent kit（Molecular Probes，Invitrogen，Eugene，OR，USA）was used to extract total RNA from the ipsilateral hemisphere according to the manufacturer’s protocol at 8and 21 days after induction of ischemia.Real-time PCR was performed using Exicycler?96 quantitative PCR Amplifier（BIONEER，Daejeon，Korea）.The primers for TGF-b1and Smad3 were purchased from the Applied Biosystems，and theβ-actin expression level was used as the internal reference.The primer sequences are listed in Table 1.The reaction condition was set at 95°C for 10 minutes，40 cycles at 95°C for 10 seconds，60°C for 20 seconds，and 72°C for30 seconds.The expression levels of target genes were normalized to those of the housekeeping geneβ-actin and were calculated using 2-ΔΔCT method（Bachman et al.，2013）.