《Table 2 Primers of real-time quantitative polymerase chain reaction》

《Table 2 Primers of real-time quantitative polymerase chain reaction》   提示：宽带有限、当前游客访问压缩模式
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Spinal cord samples were immediately removed after rats were sacrificed at 1 hour，8 hours，and 3 days after SCI and frozen on dry ice powder.Spinal cord segments were homogenized in ice-cold Trizol.Chloroform was added，and the aqueous phase was collected after centrifugation.RNA extraction was done according to the manufacturer’s protocol（Qiagen RNeasy Mini，Qiagen，Valencia，CA，USA）.RNA quality was detected using Agilent Bioanalyzer（Agilent，Palo Alto，CA，USA）in that all samples should show sharp ribosomal RNA bands.One mg of total RNA wasused for first-strand cDNA production with Super Script II reverse transcriptase（Invitrogen，Carlsbad，CA，USA）primed by Oligo dT.The real-time quantitative polymerase chain reaction（RT-qPCR）was performed on an Applied Biosystems 7900HT machine，and 10 ng of cDNA，50 nM of primers，and SYBR Green master mix（Applied Biosystems，Foster City，CA，USA）was used.Expression levels of each target gene were normalized to the mean critical threshold（CT）values of theβ-actin housekeeping gene using the 2-ΔΔCt method（Livak and Schmittgen，2001）to calculate the relative amount of RNA.The primers used are shown in Table 2.

图表编号XD0040611200    严禁用于非法目的
绘制时间2019.03.01
作者Xue Yao、Yan Zhang、Jian Hao、Hui-Quan Duan、Chen-Xi Zhao、Chao Sun、Bo Li、Bao-You Fan、Xu Wang、Wen-Xiang Li、Xuan-Hao Fu、Yong Hu、Chang Liu、Xiao-Hong Kong、Shi-Qing Feng
绘制单位Department of Orthopedics, Tianjin Medical University General Hospital、State Key Laboratory of Medicinal Chemical Biology, Nankai University、International Science and Technology Cooperation Base of Spinal Cord Injury、Department of Orthopedics, Tianjin Med
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