《Table 1 Primer sequences》
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《ESE1 expression correlates with neuronal apoptosis in the hippocampus after cerebral ischemia/reperfusion injury》
siRNA:Small interference RNA.
For a more detailed understanding of ESE1 function in cerebral I/R and to verify our assumption，three ESE1 siRNAs were established（Table 1）to knock down ESE1 in PC12cells.Western blot analysis showed that siRNA-ESE1，especially siESE1#3，strongly reduced ESE1 abundance（Figure7A and B）.MTT assays demonstrated that cell viability was remarkably lower in the CoCl2 group than in the control group（Figure 7E）.Consistent with the western blot results，knock-down of ESE1，especially siESE1#3，increased PC12cell viability compared with the CoCl2 group（Figure 7E）.Meanwhile，compared with the control and non-specific siRNA groups，ESE1 knockdown resulted in significant down-regulation of active caspase-3 and p-p65 levels in PC12 cells stimulated by CoCl2（Figure 7C and D），indicating that ESE1 inhibition might alleviate neuronal apoptosis.
| 图表编号 | XD0040614200 严禁用于非法目的 |
|---|---|
| 绘制时间 | 2019.05.01 |
| 作者 | Hai-Long Yu、Liang-Zhu Wang、Ling-Ling Zhang、Bei-Lei Chen、Hui-Juan Zhang、Yu-Ping Li、Guo-Dong Xiao、Ying-Zhu Chen |
| 绘制单位 | Clinical Medical College of Yangzhou University、Department of Neurology, Northern Jiangsu People's Hospital、Institute of Neuroscience, Northern Jiangsu People's Hospital、Drum Tower Hospital, Medical School of Nanjing University、Dalian Medical University |
| 更多格式 | 高清、无水印（增值服务） |
