《Table 1 Primer sequences》
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《Effect of lentiviral vector-mediated overexpression of hypoxia-inducible factor 1 alpha delivered by pluronic F-127 hydrogel on brachial plexus avulsion in rats》
In HIF-1αprimer sequences，lowercase letters represent lentiviral vector gene sequence and uppercase letters represent HIF-1αgene sequence.HIF-1α:Hypoxia-inducible factor 1α；F:forward primer；R:reverse primer.
To detect HIF-1αmRNA expression，qRT-PCR analysis was performed 1 week after surgery.According to the manufacturer’s instructions，total RNA was extracted from C5–7 spinal segments using Trizol（Thermo Fisher Scientific）.cDNA was reverse transcribed using the PrimeScript RT reagent Kit（TaKaRa，Tokyo，Japan）.qRT-PCR was performed using SYBR Premix Ex Taq（TaKaRa）.Relative mRNA expression from each sample was normalized toβ-actin levels.Gene expression analysis was performed by the 2–??Ct method（EL-Badawy et al.，2017）.Primer sequences of HIF-1αandβ-actin are listed in Table 1.
| 图表编号 | XD0040617400 严禁用于非法目的 |
|---|---|
| 绘制时间 | 2019.06.01 |
| 作者 | Tao Wang、Li-Ni Zeng、Zhe Zhu、Yu-Hui Wang、Lu Ding、Wei-Bin Luo、Xiao-Min Zhang、Zhi-Wei He、Hong-Fu Wu |
| 绘制单位 | Institute of Stem Cells and Regenerative Medicine,Department of Physiology,Guangdong Medical University、Department of Surgery,the Third Hospital of Guangdong Medical University (Longjiang Hospital of Shunde District)、Institute of Stem Cells and Regenerati |
| 更多格式 | 高清、无水印（增值服务） |
