《Table 1 Primer sequences》

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《Effect of H_2O_2 on Growth of Collard( Brassis oleracea L. ) Seedlings Under Salt Stress》

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Collard seeds were sterilized and rinsed for several times with distilled water，and water on the seeds was absorbed with filter paper.The treated seeds were placed in a petri dish with filter paper，and cultured in a greenhouse[temperature:（24±2）℃，light/darkness:16 h/8 h，illuminance:2 000 lx].When the radicles of collard were 3 to 5 mm long，seedlings with uniform radicles were selected and potted.When seedlings grew to a length of about 10 cm，they were placed in a Hoagland solution and pre-cultured in a greenhouse for 3 d.Three days later，the seedlings were divided into four treatments:control（CK）:Hoagland solution，salt treatment（N）:100 mmol/L Na Cl+Hoagland solution，Na Cl+H2O2treatment（N+H）:100 mmol/L Na Cl+0.05 mmol/L H2O2+Hoagland solution，and Na Cl+DMTU treatment（N+DMTU）:100 mmol/L Na Cl+5 mmol/L DMTU+Hoagland solution.After 6 h of treatment，analysis and determination of SOD，CAT and APX activity and gene expression（SOD，GenBank:JQ321587.1；CAT，Gen Bank:JF720325.1；APX，GenBank:XM＿013733887.1）were performed.Total RNA in leaves was extracted by guanidine thiocyanate method，and ABI7700 was used for quantitative PCR with actin（Gen Bank accession number:AF111812.1）as the internal reference（Table 1）according to reference[15].Each treatment had three replicates.In order to maintain the consistency of treatment，the treatment liquid was changed every day during the treatment.After 48 h of treatment，the growth rate of collard seedlings was determined，and the dry weight，fresh weight and relative water content were determined，according to reference[16].