《Table 1 Sequence-specific primer list》
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《Extracellular ADP facilitates monocyte recruitment in bacterial infection via ERK signaling》
BMDMs，PEMs and RAW 264.7 cells were stimulated with different concentrations of ADP for 3 h，and total RNA was isolated using TRIzol reagent（Life Technologies）according to the manufacturer’s protocol.Complementary DNA as synthesized with 500 ng RNA using a reverse transcription kit（Takara）according to the manufacturer’s instructions and then used for real-time（RT）-quantitative polymerase chain reaction（qPCR）analysis with SYBR Premix Ex Taq（Takara）.The data were analyzed using the Stratagene Mx3005P Real-Time qPCR System（Agilent Technologies，Santa Clara，CA，USA）.The housekeeping genesβ-actin and GAPDH were used to normalize the transcriptional levels of target genes，and the relative expression was calculated using theΔΔCtmethod.The sequence-specific primers are listed in Table 1.
| 图表编号 | XD0010729400 严禁用于非法目的 |
|---|---|
| 绘制时间 | 2018.01.01 |
| 作者 | Xiaoyu Zhang、Juliang Qin、Junyan Zou、Zhangsheng Lv、Binghe Tan、Jueping Shi、Yihan Zhao、Hua Ren、Mingyao Liu、Min Qian、Bing Du |
| 绘制单位 | Shanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences and School of Life Sciences, East China Normal University、Shanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences and School of Life Sciences, East Chi |
| 更多格式 | 高清、无水印（增值服务） |
