《Table 1 Primer sequence》
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《GPER agonist G1 suppresses neuronal apoptosis mediated by endoplasmic reticulum stress after cerebral ischemia/reperfusion injury》
Caspase-12:Cysteinyl aspartate specific proteinase 12；CHOP:C/EBPhomologous protein；GRP78:glucose-regulated protein 78.
Twenty-four hours after I/R，the hippocampal CA1 region was taken from six rats of each group for quantitative real-time polymerase chain reaction（qRT-PCR）to determine mRNA expression levels of glucose-regulated protein（GRP78），caspase-12，and C/EBP homologous protein（CHOP）.Total RNA was extracted from brain samples using a brain tissue RNA kit（Sangon Biotech，Shanghai，China）according to the manufacturer’s instructions.Isolated RNA was used for cDNA synthesis using a reverse transcription system.PCR analysis used the real-time SYBR Green PCR technology and primers shown in Table 1.A thresholding method was performed to analyze relative gene expression.Each sample was measured thrice.The 2-??Ct method was used to express relative mRNA levels（Yuan et al.，2018）.
| 图表编号 | XD0040618700 严禁用于非法目的 |
|---|---|
| 绘制时间 | 2019.07.01 |
| 作者 | Zi-Wei Han、Yue-Chen Chang、Ying Zhou、Hang Zhang、Long Chen、Yang Zhang、Jun-Qiang Si、Li Li |
| 绘制单位 | Department of Physiology,Medical College of Shihezi University、Key Laboratory of Xinjiang Endemic and Ethnic Disease,Shihezi University School of Medicine、Department of Physiology,Medical College of Shihezi University、Key Laboratory of Xinjiang Endemic an |
| 更多格式 | 高清、无水印（增值服务） |
