《Table 1 Plasmids used in this studya)》

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《Rational design for fungal laccase production in the model host Aspergillus nidulans》

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a) pXX，plasmid.

To assess laccase gene expression with different promoters，we designed and constructed the expression vector with the pslcc gene and different auxotroph markers matched with the different host strains.First，we constructed the tool vectors including pYWL14（with the gpdA promoter，TrpC terminator and AppyrG），pYWL93.4（with the gpdA promoter，TrpC terminator and AfpyroA）and pYWL94.8（with the gpdA promoter，TrpC terminator and AfriboB）（Table 1） .Then，we cloned the pslcc gene expression vectors for A.nidulans，including pYWL15（with the gpdA promoter，TrpC terminator and AppyrG），pYWL17.1（with the pslcc promoter and terminator and AppyrG），pYWL53.1（with the aflR promoter，pslcc terminator and AppyrG），pYWL55.3（with the aflR promoter，pslcc terminator and AfriboB），pYWL97.2（with the gpdA promoter，TrpC terminator and AppyrG），pYWL99.2（with the pslcc promoter and terminator and AfpriboB）and pYWL100.1（with the pslcc promoter and terminator and AfpyroA）（Table 1） .Finally，the pslcc expression cassette with its own promoter and terminator was cloned into the vector containing the AMA1（autonomous maintenance in Aspergillus）sequence（Alek-senko and Clutterbuck，1997）to obtain plasmid p YWL16（Table 1）.