《Table 4 Number of loci in tested soybean populations with a significant or an extremely difference

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《Effects of Seed Vitality and Regeneration on Genetic Integrity in Soybean by SSR Markers》

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As shown in Table 4，the number of loci in populations G0-2，G0-3 and G0-4 of the original materials（G0）with significant or extremely significantly differences in allele frequency from the control population G0-1，decreased with the decrease of vitality.Specifically，population G0-4 with a germination rate of 79.0%had the most loci with a significant or an extremely significant difference from the control population，10 with a significant and four with an extremely significant difference，respectively，and population G0-3 with a germination rate of 81.0%was next to it，having eight loci with a significant and one locus with an extremely significant difference from the control population，respectively，indicating that the decline in vitality significantly affected the allele frequency distribution in soybean germplasm material populations.There were no loci in generations G1-1 and G2-1 reproduced from population G0-1 with a significant difference from the control population，indicating that soybean germplasm materials with a germination rate of 98.0%showed hardly any difference from the control population in allele frequency of each locus after two times of reproduction.Populations G1-2 and G2-2 reproduced from population G0-2 were significantly higher than population G0-2 in number of loci with a significant or very significant difference from the control population.Specifically，population G1-2 had three loci with a significant and one locus with an extremely significant difference from the control population，respectively，and population G2-2 had three loci with a significant and two loci with an extremely significant difference from the control population，respectively.The first reproduced generations G1-3 and G1-4 and the second reproduced generations of populations G0-3 and G0-4 had more loci with a significant or an extremely significant difference from the control population，and the second reproduced generation had more loci with a significant or an extremely significant difference from the control population than corresponding first reproduced generation，indicating that soybean germplasm materials with germination rates of95.0%，81.0%，and 79.0%and their reproduced progeny populations were different from the control population in allele frequency on each loci，and the lower the vitality level，the greater the difference.The above results indicate that the decline in vitality has a higher effect on allele frequency distribution of soybean germplasm materials than reproduction generation.