《Table 1 Tested soybean germplasm populations》

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《Effects of Seed Vitality and Regeneration on Genetic Integrity in Soybean by SSR Markers》

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The seeds of soybean variety Zhonghuang 18 were provided by Institute of Crop Sciences，Chinese Academy of Agricultural Sciences.In 2001，the water content of the seeds was determined to be 8%-9%，after harvest in field.In February，2002，the seeds were sealed in aluminum foil bags and subjected to artificial aging treatment（0，112，154 and 196 d）at（40±2）°C，obtaining seed populations as the original materials（G0）at four different vitality levels（G0-1，G0-2，G0-3 and G0-4），and seeds free of artificial aging treatment（G0-1）served as the control（CK）.In 2003，the above four seed populations were planted separately in field，obtaining four seed populations（G1）of the first reproduced generation（G1-1，G1-2，G1-3 and G1-4）.In 2006，the seed populations of the first reproduced generation were seeded in field，obtaining four seed populations（G2）of the second reproduced generation（G2-1，G2-2，G2-3 and G2-4）（Table 1） .The reproduction was carried out at the Dishang Experimental Station of Institute of Food and Oil Crops，Hebei Academy of Agricultural and Forestry Sciences，in Shijiazhuang City.The field reproduction adopted randomized block design，with population as the treatment unit，and each treatment had three replicates，each of which was sown with 200 seeds.The reproduction and regeneration were according to Technical Regulation on Characterization and Documentation for Crop Germplasm Resources[16]，and seeds were harvested according to the mixed method.All harvested seeds were air-dried and stored at 4℃for testing.