《Table 3 Genetic composition of the tested soybean germplasm population by SSR》

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《Effects of Seed Vitality and Regeneration on Genetic Integrity in Soybean by SSR Markers》

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NA=No.of Alleles；K=No.of polymorphic loci；P=percentage of polymorphic loci；A=No.of alleles per locus；Ae=No.of effective alleles per locus；H=Genetic diversity index；I=Shannon's information index*:Significant at 5%level；**:Significant at 1%level.

It could be seen from Table 3 that the number of effective alleles per locus in each population was not significantly different from that of the control population.For populations G0-2，G0-3and G0-4 among the original materials（G0），and their reproduced populations G1-2，G1-3，G1-4 and G2-2，G2-3，G2-4，the genetic diversity parameters were lower than the control population G0-1，and with the prolongation of the aging time，vitality level and the genetic diversity parameters both became lower.The results of the t test showed that for the reproduced generations G1-1 and G2-1 of the control population，various genetic diversity parameters also most had no differences from the control group G0-1，indicating that the genetic structure of the population with a regeneration germination rate of 98.0%was better maintained after two times of reproduction.There were no significant differences in the genetic diversity parameters A，H and I between group G0-2 and the control population，while its first reproduced generation G1-2 and second reproduced generation G1-2 showed significant differences from the control population in A，indicating that the progeny populations of the population with a regeneration germination rate of 95.0%had a significant change in the number of alleles per locus due to the decline in the regeneration germination rate of the parental generation.The three genetic diversity parameters A，H and I of populations G0-3 and G0-4 were very significantly different from the control population G0-1.The three genetic diversity parameters A，H and I of the progeny populations of populations G0-3 and G0-4，i.e.，populations G1-3，G2-3 and G1-4，G2-4，were significantly different，or very significantly different from the control population，but these parameters were slightly higher than populations G0-3 and G0-4，respectively，indicating that for the populations with regeneration germination rates of 81.0%and 79.0%，respectively，due to the decline in vitality level，their own genetic diversity and the genetic diversity of their progeny populations were lower than that of the control population.The above results indicate that the decline in vitality has a higher effect on the population genetic structure of soybean germplasms than reproduction generation.